Complementation of the rev gene mutation among human and simian lentiviruses.
AUTOR(ES)
Sakai, H
RESUMO
The functional exchangeability of the rev gene was assessed in transient transfection experiments by using in vitro-constructed rev and gag mutants of the following three primate lentiviruses: human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus from the African green monkey (SIV AGM). Cotransfection into SW480 cells of the rev and gag mutants derived from the DNA of each infectious virus resulted in the generation of progeny particles as determined by reverse transcriptase assay. rev gene mutants of HIV-2 and SIV AGM were also complemented by all gag mutants derived from the three viruses. In contrast, no evidence of complementation was obtained following cotransfection of the HIV-1 rev mutant and the gag mutant of HIV-2 or SIV AGM.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=249380Documentos Relacionados
- Vpr-induced cell cycle arrest is conserved among primate lentiviruses.
- Analysis of antibody responses to phenotypically distinct lentiviruses.
- Replication properties of dUTPase-deficient mutants of caprine and ovine lentiviruses.
- Human T-cell lymphotropic virus type III shares sequence homology with a family of pathogenic lentiviruses.
- Evidence for interference, coinfections, and intertypic virus enhancement of infection by ovine-caprine lentiviruses.