Complete nucleotide sequence of a French bean storage protein gene: Phaseolin
AUTOR(ES)
Slightom, Jerry L.
RESUMO
The complete nucleotide sequences of the gene and the mRNA coding for a specific phaseolin type French bean major storage protein have been determined. Comparison of these sequences reveals a phaseolin gene structure consisting of 80 base pairs (bp) of 5′ untranslated DNA, 1,263 bp of protein-encoding DNA which is interrupted by five intervening sequences (IVS1, 72 bp; IVS2, 88 bp; IVS3, 124 bp; IVS4, 128 bp; and IVS5, 103 bp), and 135 bp of 3′ untranslated DNA. Sequences characteristic of eukaryotic promoters “CCAAT” and “TATA” are present in the 5′ flanking DNA, and the eukaryotic poly(A) addition signal A-A-T-A-A-A occurs 16 bp before the first nucleotide of poly(A). The derived amino acid sequence yields an amino acid composition and a molecular weight compatible with those found for the β-type phaseolin protein. Two regions that probably serve as carbohydrate-peptide linkage recognition sites have been identified. A region of highly hydrophobic amino acids at the NH2 terminus of the protein suggests the presence of a signal peptide in the newly synthesized phaseolin protein.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393717Documentos Relacionados
- Complete nucleotide sequence of gene for sex-specific storage protein of Bombyx mori.
- Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein.
- Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.
- Analysis of a cloned colicin Ib gene: complete nucleotide sequence and implications for regulation of expression.
- Analysis of a cloned colicin Ib gene: complete nucleotide sequence and implications for regulation of expression
