Complete nucleotide sequence of the murine H-2Kk gene. Comparison of three H-2K locus alleles.

AUTOR(ES)

Arnold, B

RESUMO

We have determined the DNA sequence of the H-2Kk gene of the mouse major histocompatibility complex (MHC). Comparison on the nucleotide and protein level of three H-2K alleles (Kk, Kb and Kd) reveals a high degree of homology, in particular between the Kb and Kk alleles. Differences between the two latter antigens are almost exclusively confined to the alpha 1 and alpha 2 domains. At nine positions in the extracellular part of the molecules we have found allele-specific amino acids. Interestingly, 78% of these residues are either polar or carry hydroxyl-groups. This makes it likely that they are exposed on the surface of the molecules and might then be part of antigenic determinants. We have also identified potentially allele-specific nucleotide sequences of the K genes which might be used as specific DNA probes.

Documentos Relacionados

• Distribution and mobility of murine histocompatibility H-2Kk antigen in the cytoplasmic membrane.
• Complete nucleotide sequence of the Bacillus subtilis dnaK gene.
• Structure of the heavy chain of the H-2Kk histocompatibility antigen.
• Dilute-coat-color locus of mice: nucleotide sequence analysis of the d+2J and d+Ha revertant alleles.
• The partial amino-acid sequence of an H-2K molecule.