Comportamento de mutantes de endosperma de milho (zea mays L.) em relação ao fuzarium moniliforme Sheld

AUTOR(ES)
DATA DE PUBLICAÇÃO

1982

RESUMO

A comparative study involving 11 different endosperm mutants of maize and their correspondent normal counterparts was carried out to study the effect of endosperm mutant genes on pathogenicity of F. moniliforme. The following mutants were studied: Shrunken (sh), shrunken-2 (sh2), floury (fl), brittle (bt) brittle-2 (bt2), waxy (wx), dull (du), sugary (su), sugary-2 (su2), opaque-2 (o2) and amylose extender (ae). Those genes were introduced in several synthetic varieties through a backcross procedure and were studied in S5 inbred lines derived from them. In a preliminary step it was studied the presence of variants among cultures of F. moniliforme isolated from corn seeds. It was found that the pathogen occur in nature with different types being predominant the purple type. Apparently the types occur in nature by heterocaryosis. A randomized complete block experiment involving 11 inbred lines segregating only for the endosperm gene, with 5 replications, was planted at the experimental field. At flowering the 10 m long plots were selfed and the segregating ears were artificially inoculated with spores from a purple colony of F. moniliforme. The study of seed pathogenicity was carried out by the isolation of the fungus from 11 endosperm and their normal kernels of the segregating ears. Mutants and their normal kernels of the segregating ears. Two tests were made. One, with deep-freezing treatment at -19ºC and the other with intact seeds

ASSUNTO(S)

milho - reprodução

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