Conformational transitions of the phosphodiester backbone in native DNA: two-dimensional magic-angle-spinning 31P-NMR of DNA fibers.
AUTOR(ES)
Song, Z
RESUMO
Solid-state 31P-NMR is used to investigate the orientation of the phosphodiester backbone in NaDNA-, LiDNA-, MgDNA-, and NaDNA-netropsin fibers. The results for A- and B-DNA agree with previous interpretations. We verify that the binding of netropsin to NaDNA stabilizes the B form, and find that in NaDNA, most of the phosphate groups adopt a conformation typical of the A form, although there are minor components with phosphate orientations close to the B form. For LiDNA and MgDNA samples, on the other hand, we find phosphate conformations that are in variance with previous models. These samples display x-ray diffraction patterns that correspond to C-DNA. However, we find two distinct phosphate orientations in these samples, one resembling that in B-DNA, and one displaying a twist of the PO4 groups about the O3-P-O4 bisectors. The latter conformation is not in accordance with previous models of C-DNA structure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1181053Documentos Relacionados
- The 31P-NMR spectrum of the dodecamer d(GACGATATCGTC).
- 31P-NMR Spectroscopy of Roots of Intact Corn Seedlings 1
- A 31P-NMR study of the interaction of Mg2+ ions with nucleoside diphosphates.
- Study of phospholipid structure by 1H, 13C, and 31P dipolar couplings from two-dimensional NMR.
- Compartmentation of Nucleotides in Corn Root Tips Studied by 31P-NMR and HPLC 1