Constitutive production of alpha and beta interferons in mutant human cell lines.
AUTOR(ES)
McKendry, R
RESUMO
Alpha and beta interferons control expression of a selectable marker in the human hypoxanthine phosphoribosyltransferase-negative cell line 2fTGH, in which transcription of gpt is regulated by the upstream region of an interferon-responsive human gene. Selection of mutagenized 2fTGH cells in hypoxanthine-aminopterin-thymidine medium yielded mutants in one recessive (C1) and two dominant (C2 and C3) complementation groups. The mutants constitutively expressed low levels of beta interferon (C1), alpha interferon (C2), or both (C3).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=236917Documentos Relacionados
- Constitutive production of macrophage colony-stimulating factor by human ovarian and breast cancer cell lines.
- Differential expression of alpha- and beta-globin genes in erythroleukemic cell lines.
- Homozygous deletion of the alpha- and beta 1-interferon genes in human leukemia and derived cell lines.
- Spontaneous production of gamma interferon and induced production of beta interferon by human T-lymphoblastoid cell lines.
- Isolation and characterization of a new mutant human cell line unresponsive to alpha and beta interferons.