Construção de sistema que permite a ancoragem de proteína recombinante à superfície celular de levedura. / Construction of a system that allows anchoring of recombinant protein to the cell surface of yeast.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Cell surface display systems have being developed for expression of heterologous proteins anchored to the cell surface of microorganisms. Several applications of these systems have been reported, including employment as whole-cell biocatalysts, development of vaccines and cellular biosorvents. In this work it was developed a system that allows the anchoring of the Aspergillus awamori glucoamylase protein to the cell wall surface of the yeast Saccharomyces cerevisiae. The gene encoding glucoamylase with its secretion signal was fused to the gene fragment encoding the C-terminal region of Flo1 protein, used as an anchor (CG*FC fragment). Yeast cells were transformed with hybrid CG*FC fragment and transformants were able to degrade starch and release glucose. Glucoamylase activity was not detected in the culture medium, but only in sedimented cells. These results demonstrate that glucoamylase was anchored to the cell wall of the new yeast recombinant strain.

ASSUNTO(S)

anchor flo1p saccharomyces cerevisiae biotechnology sistema de ancoragem de proteínas anchoring protein system superfície celular glicoamilase cell surface Âncora flo1p glucoamylase saccharomyces cerevisiae biotecnologia

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