Construction and Characterization of Mutations at Codon 751 of the Escherichia coli gyrB Gene That Confer Resistance to the Antimicrobial Peptide Microcin B17 and Alter the Activity of DNA Gyrase

AUTOR(ES)

del Castillo, Francisco J.

FONTE

American Society for Microbiology

RESUMO

Microcin B17 is a peptide antibiotic that inhibits DNA replication in Escherichia coli by targeting DNA gyrase. Previously, two independently isolated microcin B17-resistant mutants were shown to harbor the same gyrB point mutation that results in the replacement of tryptophan 751 by arginine in the GyrB polypeptide. We used site-directed mutagenesis to construct mutants in which tryptophan 751 was deleted or replaced by other amino acids. These mutants exhibit altered DNA gyrase activity and different levels of resistance to microcin B17.

Documentos Relacionados

• Quinolone resistance-determining region in the DNA gyrase gyrB gene of Escherichia coli.
• Quinolone resistance mutations in the DNA gyrase gyrA and gyrB genes of Staphylococcus aureus.
• The peptide antibiotic microcin B17 induces double-strand cleavage of DNA mediated by E. coli DNA gyrase.
• Mutations in Bartonella bacilliformis gyrB Confer Resistance to Coumermycin A1
• gyrA and gyrB mutations in quinolone-resistant strains of Escherichia coli.