Construction and Consequences of Directed Mutations Affecting the Hemin Receptor in Pathogenic Corynebacterium Species

AUTOR(ES)

Schmitt, Michael P.

FONTE

American Society for Microbiology

RESUMO

Genes encoding an ATP-binding cassette transporter system involved in hemin iron utilization from Corynebacterium ulcerans were cloned and characterized. The genes are homologous to a hemin transport system previously identified in Corynebacterium diphtheriae. Disruption of the hmuT gene, which encodes the putative hemin receptor, resulted in greatly reduced ability of C. ulcerans to use hemin or hemoglobin as an iron source. Inactivation of hmuT in C. diphtheriae by site-specific recombination had no effect on hemin utilization, which suggests that C. diphtheriae has an additional system for transporting hemin.

Documentos Relacionados

• The consequences of pathogenic mutations to the human prion protein
• Directed mutations affecting spectroscopic and electron transfer properties of the primary donor in the photosynthetic reaction center
• Hemin-dependent growth stimulation and cytochrome synthesis in Corynebacterium pyogenes.
• Construction and Characterization of Transposon Insertion Mutations in Corynebacterium diphtheriae That Affect Expression of the Diphtheria Toxin Repressor (DtxR)
• Improved enzymatic in vitro reactions in the gapped duplex DNA approach to oligonucleotide-directed construction of mutations.