Construction and properties of replication-competent murine retroviral vectors encoding methotrexate resistance.
AUTOR(ES)
Stuhlmann, H
RESUMO
A series of replication-competent Moloney murine leukemia virus vectors was constructed in which each vector contained a mutant dihydrofolate reductase (DHFR) cDNA insert in the U3 region of the viral long terminal repeat. Two of the resulting viruses, MLV (murine leukemia virus) DHFR*-5 and MLV DHFR*-7, were able to stably transfer methotrexate resistance to infected fibroblast cells upon multiple rounds of virus replication and in the absence of drug selection. Cell lines producing recombinant virus with high titers were established, which indicated that the insert did not grossly interfere with viral replication functions. These vectors should be useful for introducing and expressing foreign genes in vivo in tissues and whole animals in which virus spread is needed for efficient infection.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=362150Documentos Relacionados
- Construction and Characterization of a Replication-Competent Retroviral Shuttle Vector Plasmid
- Regulated Gene Expression in the Chicken Embryo by Using Replication-Competent Retroviral Vectors
- Construction and characterization of replication-competent simian immunodeficiency virus vectors that express gamma interferon.
- Properties of Replication-Competent Vesicular Stomatitis Virus Vectors Expressing Glycoproteins of Filoviruses and Arenaviruses
- Construction of replication-competent Herpesvirus saimiri deletion mutants.