Construction and Synchronization of dnaA Temperature-Sensitive Mutants of Streptomyces
AUTOR(ES)
Lee, Li-Fong
FONTE
American Society for Microbiology
RESUMO
Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39°C replication in the mutants ceased in about 90 min but resumed on return to 30°C. This allowed manipulations to achieve replication synchronization.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=134811Documentos Relacionados
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