Construction of a detailed molecular map of the mouse X chromosome by microcloning and interspecific crosses.

AUTOR(ES)

Brockdorff, N

RESUMO

A large number of microclones obtained by microdissection of the mouse X chromosome have been mapped using an interspecific Mus domesticus/Mus spretus cross. Clones displaying close linkage to a number of loci of known phenotype but unknown gene product, such as mdx (X-linked muscular dystrophy), have been obtained. Over a central 30 cM span of the mouse X chromosome, 17 clones have been mapped and ordered at a sufficient density to contemplate the complete physical mapping of this region that will aid in the isolation of a number of unidentified genes. Some of the mapped microclones detect moderately repetitive sequences that were clustered in several discrete regions of the mouse X chromosome.

Documentos Relacionados

• Microdissection and microcloning of the mouse X chromosome.
• Mapping genes in the mouse using single-strand conformation polymorphism analysis of recombinant inbred strains and interspecific crosses.
• Detailed ordering of markers localizing to the Xq26-Xqter region of the human X chromosome by the use of an interspecific Mus spretus mouse cross.
• A Genetic Linkage Map of Mouse Chromosome 10: Localization of Eighteen Molecular Markers Using a Single Interspecific Backcross
• Mapping of the mouse X chromosome using random genomic probes and an interspecific mouse cross.