Construction of a vector, pRSVcatamb38, for the rapid and sensitive assay of amber suppression in human and other mammalian cells.

AUTOR(ES)

Burke, J F

RESUMO

We describe the generation of an amber mutation in the chloramphenicol acetyltransferase (cat) gene of the mammalian cell transfection vector pRSVcat (Gorman et.al. (1982), Proc.Natl.Acad.Sci. 79 6777-6791). We have demonstrated the in vivo suppression of this amber mutation in monkey and human cells by co-transfection with a synthetic Xenopus suppressor tRNATyr under the control of the late SV40 promoter. The vector, pRSVcatamb38, may be used to quantitate amber suppression in various mammalian cells.

Documentos Relacionados

• A rapid and sensitive assay system for bacterial gpt activity in transfected mammalian cells.
• A sensitive and rapid assay for homologous recombination in mosquito cells: impact of vector topology and implications for gene targeting
• Tetracycline-Regulated Suppression of Amber Codons in Mammalian Cells
• A ubiquitous mammalian expression vector, pHMG, based on a housekeeping gene promoter.
• Construction of luciferase reporter bacteriophage A511::luxAB for rapid and sensitive detection of viable Listeria cells.