Contribution of the Zinc Finger to Zinc and DNA Binding by a Suppressor of Posttranscriptional Gene Silencing

AUTOR(ES)

van Wezel, Rene

FONTE

American Society for Microbiology

RESUMO

The zinc finger C36-X1-C38-X7-C46-X6-H53 of the nuclearly localized C2 protein of Tomato yellow leaf curl virus China is involved in pathogenicity and suppression of posttranscriptional gene silencing (PTGS). Here, we demonstrate that the zinc finger is indispensable for the C2 protein to bind zinc and DNA. Mutation of cysteine residue C36, C38, or C46 reduced the zinc and DNA binding capacity of C2 protein. When expressed from potato virus X, all three mutants, C2-C36R, C2-C38N, and C2-C46I, tagged with a green fluorescent protein (GFP) were still capable of transporting GFP into but aggregated abnormally in nuclei. Our data establish that zinc- and DNA-binding activity correlates with C2-mediated pathogenesis and PTGS suppression.

Documentos Relacionados

• RNA binding by the Wilms tumor suppressor zinc finger proteins.
• Virus-encoded suppressor of posttranscriptional gene silencing targets a maintenance step in the silencing pathway
• Functional Characterization of the Nuclear Localization Signal for a Suppressor of Posttranscriptional Gene Silencing
• Defective Interfering RNA Hinders the Activity of a Tombusvirus-Encoded Posttranscriptional Gene Silencing Suppressor
• Cooperative, non-specific binding of a zinc finger peptide to DNA.