Control of adenovirus early gene expression: accumulation of viral mRNA after infection of transformed cells.
AUTOR(ES)
Persson, H
RESUMO
We studied the accumulation of viral mRNA in the presence of inhibitors of protein synthesis in an adenovirus type 5-transformed cell line (line 293 cells). An analysis of the endogenous viral mRNA's and proteins revealed that only early regions 1A and 1B were expressed in uninfected 293 cells. However, viral mRNA's from early regions 2, 3, and 4, as well as mRNA's from early regions 1A and 1B, accumulated in 293 cells after infection with adenovirus type 2. Cells treated with anisomycin before infection showed a drastic enhancement of mRNA from early region 4 compared with drug-free controls, This increase in viral mRNA was detected by using filter hybridization, S1 endonuclease mapping, and in vitro translation. The rate of transcription of early region 4 nuclear RNA also increased significantly in the presence of anisomycin. In contrast to the results with early region 4, the levels of cytoplasmic mRNA's from early regions 2 and 3 did not increase in cells treated with inhibitors. These results suggested that multiple virus encoded controls operate on the early regions of the adenovirus genome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=256636Documentos Relacionados
- Two "early" mRNA species in adenovirus type 2-transformed rat cells.
- Transcription of the genome of adenovirus type 12. I. Viral mRNA in abortively infected and transformed cells.
- Control of adenovirus early gene expression: posttranscriptional control mediated by both viral and cellular gene products.
- Sequence-mediated regulation of adenovirus gene expression by repression of mRNA accumulation.
- Vero cells injected with adenovirus type 2 mRNA produce authentic viral polypeptide patterns: early mRNA promotes growth of adenovirus-associated virus.
