Control of transcription initiation in vitro requires binding of a transcription factor to the distal promoter of the ovalbumin gene.
AUTOR(ES)
Pastorcic, M
RESUMO
We used a cell-free HeLa cell transcription system to identify and characterize transcription factors and the promoter elements that they recognize in RNA polymerase II-transcribed genes. Deletion of the region (-71 to -83) containing the GTCAAA direct repeat resulted in a marked decrease of specific transcription of the ovalbumin gene; transcription could be competed with DNA fragments containing this sequence. Furthermore, DNase I footprinting identified a protein-binding site including this direct repeat with crude extracts and one of the partially purified protein fractions required for transcription. We propose that a soluble factor activates transcription through binding to the direct repeat of GTCAAA sequence upstream from the ovalbumin gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=367845Documentos Relacionados
- Proximal and distal domains that control in vitro transcription of the adenovirus IVa2 gene.
- A nucleosome positioned in the distal promoter region activates transcription of the human U6 gene.
- Identification of potato nuclear proteins binding to the distal promoter region of the proteinase inhibitor II gene.
- Specific 5′ flanking sequences are required for faithful initiation of in vitro transcription of the ovalbumin gene
- Differential binding of a S. cerevisiae RNA polymerase III transcription factor to two promoter segments of a tRNA gene.