Cooperative interaction of chicken lysozyme enhancer sub-domains partially overlapping with a steroid receptor binding site.
AUTOR(ES)
Altschmied, J
RESUMO
Expression of the lysozyme gene is a marker for the differentiation of macrophages, lysozyme transcription being gradually increased during maturation. We have analyzed the fine structure and function of two macrophage-specific enhancer elements of the chicken lysozyme gene (E-2.7 kb and E-0.2 kb). Both increase their activities upon LPS induction, both contain multiple binding sites for similar or identical nuclear factors and both can be divided into two functional modules. For the E-0.2 kb enhancer we found a synergistic activity of the modules to be dependent on their distance. Binding sites for nuclear proteins within enhancer E-0.2 kb overlap substantially with the previously identified progesterone/glucocorticoid receptor binding site, which is required for steroid induction of lysozyme transcription in the oviduct.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=318088Documentos Relacionados
- Two silencing sub-domains of v-erbA synergize with each other, but not with RXR.
- Interaction of p53 with its consensus DNA-binding site.
- DNA-methyltransferase SsoII interaction with own promoter region binding site.
- A progesterone responsive element maps to the far upstream steroid dependent DNase hypersensitive site of chicken lysozyme chromatin.
- Interaction of the v-rel protein with an NF-kappa B DNA binding site.