Coordinate expression of parietal endodermal functions in hybrids of embryonal carcinoma and endodermal cells.

AUTOR(ES)

Howe, W E

RESUMO

A derivative, FOT5, of the F9 murine embryonal carcinoma cell line which is resistant to ouabain and thioguanine was fused with a near diploid parietal endodermal cell line, PFHR9, Hybrid clones (ENEC1 to ENEC5) were isolated in HAT Medium containing ouabain at a frequency of approximately 2 x 10(-4). The DNA contents and chromosome number of the ENEC hybrids were approximately the sum of those of the parents. Five hybrid cell lines examined in detail expressed the following parietal endodermal functions: plasminogen activator activity, basement membrane proteins, and endodermal cytoskeletal proteins. Embryonal carcinoma characteristic functions (tumorigenicity, a stage specific embryonic antigen, and high alkaline phosphatase activity) were extinguished in the hybrids. No hybrid clones with embryonal carcinoma morphology were observed among 1,358 hybrid clones examined. Hybrids, propagated for over 100 generations, continued to express endodermal functions and not embryonal carcinoma functions. The coordinate expression of endodermal functions and the extinction of embryonal carcinoma functions in the ENEC hybrids suggest that the parietal endodermal cells contain diffusible activities which extinguish embryonal carcinoma functions and possibly cause the embryonal carcinoma genome to express parietal endodermal characteristics.

Documentos Relacionados

• Negative transcriptional regulatory element that functions in embryonal carcinoma cells.
• Integration-specific retrovirus expression in embryonal carcinoma cells.
• Determinants of retrovirus gene expression in embryonal carcinoma cells.
• Increased probability of expression from modified retroviral vectors in embryonal stem cells and embryonal carcinoma cells.
• Negative regulation of early polyomavirus expression in mouse embryonal carcinoma cells.