Copy Number Mutations (Cop−) of the Plasmid Containing the Replication Origin (oriC) of the Escherichia coli Chromosome: Lethal Effect of the Cop Region Cloned onto a High-Copy-Number Vector on Host Cells

AUTOR(ES)

Yamaguchi, Kazuo

RESUMO

High-copy-number mutants were isolated from an oriC plasmid. They carried insertion mutations within a region (about 470 base pairs) near the uncB gene. When a segment containing this region was cloned onto a high-copy-number plasmid, such a plasmid could be maintained as an intact form only when it was present in a lower copy number.

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