Correlação entre os ensaios de citotoxicidade em Artemia salina Leach e atividade antineoplásica sobre linhagens de células tumorais para algumas classes de produtos naturais / Correlation between the citotoxicity assays in Artemia salina Leach and antineoplasic activity on tumoral cell lines for some natural products groups

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The aim of this study was to correlate qualitative and quantitatively the lethal concentration needed for the citotoxic test on Artemia salina with the inhibitory concentration for the growth of tumoral cell lines using ten pure substances already known. Estimates for the year 2008 and also valid for the year 2009, in Brazil, show the occurrence of 466.730 new cases of cancer. It makes necessary the development of compounds that aim the treatment of this disease. The Brazilian biodiversity contributes with the identification of products with economic uses and plants are the important sources of biologically active natural products. The A. salina Leach brine shrimp assay is among the simple and fast bioassays used to evaluate the toxicity of products and plant extracts. It is considered a preliminary bioassay in citotoxicity studies and an indicative test for more specific ones like the bioassay with mammalian cell lines (antitumoral). Substances extracted from plants and belonging to the following groups of natural products were evaluated for their citotoxic activity: alkaloids, flavonoids and quinones. The A. salina eggs are hatched in artificial seawater and used in the nauplii stage to test ten substances in concentrations of 250, 125, 61.5 and 31.3 μg/mL: Boldine, Duguetine, Aloin, Emodin, Rutin, Quercetin, Lapachol, β-Lapachone, NNitrosoanonaine e N-Nitrosoxylopine. Hep2 (larynx carcinom) and B16-F10 (murine melanoma) cell lines are cultivated in 96-well plates and the viability evaluated with the sulforhodamine B stain method after 48 hours of exposure to the tested substances. The quinones were the most active among the three natural products classes evaluated in both bioassays, A. salina and antitumoral. β-Lapachone was the substance with lower IC50 for both tumoral cell lines and Duguetine, an alkaloid, the most citotoxic to A. salina. The results confirm the A. salina bioassay as an indicative of antitumoral activity although it is not possible to establish a secure quantitative correlation between ED50 and IC50 from these tests.

ASSUNTO(S)

atividade citotóxica artemia salina citotoxic activity farmacognosia srb assay quinonas flavonoids quinones alkaloids ensaio antitumoral srb flavonóides alcalóides

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