Correspondence between immunological and functional domains in the transforming protein of Fujinami sarcoma virus.
AUTOR(ES)
Stone, J C
RESUMO
Monoclonal antibodies reactive with either gag or fps portions of the wild-type Fujinami sarcoma virus transforming protein have been used to probe the structure of proteins encoded by mutant genomes constructed in vitro. The pattern of immunoreactivity suggests that the functional domain defined in genetic studies (Stone et al., Cell 37:549-558, 1984) corresponds to a discrete immunological domain in the native, wild-type Fujinami sarcoma virus protein. At least one mutation affecting both the structure and function of the proposed NH2-terminal fps-specific domain encodes a product with high specific activities in kinase assays. Furthermore, a cell line expressing high levels of this mutant protein is only moderately transformed. The striking correspondence between the immunological domain defined here and the functional domain inferred from the results of transfection experiments suggests that this non-kinase-specifying region constitutes a discrete structural as well as functional component of the viral protein.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=255055Documentos Relacionados
- Delineation of functional determinants in the transforming protein of Fujinami sarcoma virus.
- Characterization of the transforming gene of Fujinami sarcoma virus.
- Cellular localization of the transforming protein of wild-type and temperature-sensitive Fujinami sarcoma virus.
- Mapping of multiple phosphorylation sites within the structural and catalytic domains of the Fujinami avian sarcoma virus transforming protein.
- Structural and functional domains of the Rous sarcoma virus transforming protein (pp60src).