Coupling Between Bacteriochlorophyll and Membrane Protein Synthesis in Rhodopseudomonas spheroides

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RESUMO

Bacteriochlorophyll-containing membranes from Rhodopseudomonas spheroides contain proteins with estimated molecular weights of 26,000, 22,000, 19,000, and 10,000-6,000 (proteins 9, 10, 11, and 15) when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Proteins 9, 10, and 11 may be associated with the reaction center form of bacteriochlorophyll and protein 15, with the light-harvesting form. These proteins were not detected in membranes from nonpigmented wild-type cells grown with high aeration. Proteins 10, 11, and 15 were not found in mutants with blocks in bacteriochlorophyll synthesis, including strain 8-17, which is blocked at the phytolation step. Protein 9 was found in significantly reduced amounts. Apparently, synthesis of the completed bacteriochlorophyll molecule is required for the occurrence of these proteins in the membrane.

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