Cross-Regulation between a Novel Two-Component Signal Transduction System for Catabolism of Toluene in Pseudomonas mendocina and the TodST System from Pseudomonas putida
AUTOR(ES)
Ramos-González, María-Isabel
FONTE
American Society for Microbiology
RESUMO
The tmoABCDEF genes encode the toluene-4-monooxygenase from Pseudomonas mendocina KR1. Upstream from the tmoA gene an open reading frame, tmoX, encoding a protein 83% identical to TodX (todX being the initial gene in the todXFC1C2BADEGIH operon from Pseudomonas putida DOT-T1E) was found. The tmoX gene is also the initial gene in the tmoXABCDEF gene cluster. The transcription initiation point from the tmoX promoter was mapped, and the sequence upstream revealed striking identity with the promoter of the tod operon of P. putida. The tod operon is regulated by a two-component signal transduction system encoded by the todST genes. Two novel genes from P. mendocina KR1, tmoST, were rescued by complementation of a P. putida DOT-T1E todST knockout mutant, whose gene products shared about 85% identity with TodS-TodT. We show that transcription from PtmoX and PtodX can be mediated by TmoS-TmoT or TodS-TodT, in the presence of toluene, revealing cross-regulation between these two catabolic pathways.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=135474Documentos Relacionados
- rgf Encodes a Novel Two-Component Signal Transduction System of Streptococcus agalactiae
- An Essential Two-Component Signal Transduction System in Mycobacterium tuberculosis
- Two-Component Signal Transduction in Enterococcus faecalis†
- Two-Component Signal Transduction Pathways in Arabidopsis1
- Functional Analysis of the Mycobacterium tuberculosis MprAB Two-Component Signal Transduction System