Cryptic DNA-binding domain in the C terminus of RNA polymerase II general transcription factor RAP30.
AUTOR(ES)
Tan, S
RESUMO
The C terminus of mammalian transcription factor RAP30 has been found to be a cryptic DNA-binding domain strikingly similar to the C-terminal DNA-binding domain present in conserved region 4 of members of the sigma 70 family of bacterial sigma factors. This RAP30 domain shares strongest sequence similarity with the DNA-binding domain present in region 4 of Bacillus subtilis sporulation-specific sigma K. Like the region 4 DNA-binding activity of Escherichia coli sigma 70, the RAP30 C-terminal DNA binding activity is masked in intact RAP30 but is readily detectable when the RAP30 C terminus is expressed as a fusion protein. Consistent with a role for RAP30 DNA-binding activity in transcription, mutations that abolish DNA binding also abolish transcription. Therefore, RAP30 may function at least in part through the action of an evolutionarily ancient DNA-binding domain that first appeared prior to the divergence of bacteria and eukaryotes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=44906Documentos Relacionados
- The general transcription factor RAP30 binds to RNA polymerase II and prevents it from binding nonspecifically to DNA.
- Imperfect conservation of a sigma factor-like subregion in Xenopus general transcription factor RAP30.
- Inhibition of RNA polymerase II transcription in human cells by synthetic DNA-binding ligands
- Elongation factor SII-dependent transcription by RNA polymerase II through a sequence-specific DNA-binding protein.
- A DNA-binding domain of human transcription factor IIIC2.