Crystallization of tRNALeu-Synthetase from Baker's Yeast
AUTOR(ES)
Chirikjian, Jack G.
RESUMO
tRNALeu-Synthetase from baker's yeast has been crystallized from ammonium sulfate solution. The crystals are of orthorhombic symmetry, falling into space group [unk] or [unk] with a = 75.5 Å, b = 110.7 Å, and c = 124.0 Å. This unit cell contains four molecules of the native (dimeric) enzyme, with eight asymmetric units, each corresponding to the enzyme subunit. Diffraction patterns obtained for the three major projections contain reflections to spacings of less than 3.5 Å, indicating that this crystal form is amenable to structure analysis to atomic resolution.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=426765Documentos Relacionados
- Primary structure of baker's yeast tRNAVal 2b.
- Genetic Analysis of Haploids from Industrial Strains of Baker's Yeast
- Chromatin subunits from baker's yeast: isolation and partial characterization.
- Incorporation of C14-pantothenate into the fatty acid synthetase complex of growing baker's yeast.
- Phenylalanyl-tRNA synthetase from baker's yeast: role of 3'-terminal adenosine of tRNA-Phe in enzyme-substrate interaction studied with 3'-modified tRNA-Phe species.