Culture of Serum-induced Spheroplasts from Vibrio cholerae
AUTOR(ES)
Iannetta, Antoinette
RESUMO
Spheroplasts of Vibrio cholerae types Inaba, Hikojima, and Ogawa were produced in serum. An excess of lysozyme was added to expedite transformation, and CaCl2 was used as the stabilizing agent. At the optimal time for each strain, when less than 1% classical rod forms were observed microscopically, samples were plated on both conventional and modified L-form media. No growth occurred on conventional media; L-form type colonies appeared on L-form media after 2 to 6 days of incubation. L-form colonies could be subcultured on conventional media and required from four to six passages for complete reversion to classical parent forms. Reaction mixtures of Hikojima and Inaba types were passed through membrane filters (0.45 μ); L-form colonies were grown from both strains after spheroplast transformation. Appropriate controls were negative. It is suggested that the replicative particle may be filtrable.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=276668Documentos Relacionados
- The role of the liver in serum-induced hypercoagulability.
- EFFECT OF SEX HORMONES ON THE SERUM-INDUCED THROMBOSIS PHENOMENON*
- THE ROLE OF HUMAN COAGULATION FACTORS IN SERUM-INDUCED THROMBOSIS *
- Activation Product, Factor IX, Serum Thrombotic Accelerator Activity, and Serum-induced Thrombosis*
- Ras Signaling Is Required for Serum-Induced Hyphal Differentiation in Candida albicans