Curing of the killer deoxyribonucleic acid plasmids of Kluyveromyces lactis.
AUTOR(ES)
Niwa, O
RESUMO
Ultraviolet irradiation gave rise to frequent curing of killer plasmids pGKl1 and pGK12 of Kluyveromyces lactis. Almost all of the nonkillers obtained lost both plasmids, but one of them lost only pGKl1. The disappearance of pGKl1 was accompanied by the simultaneous loss of the killer activity and of the resistance to the killer factor. A new plasmid, pGKl1S, was obtained, which arose from a deletion in the central region of pGKl1. Genetic analysis suggested that pGKl1S has the killer gene lost by the deletion and the resistance gene intact and that pGKl1S shares the same replication control with pGKl1.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=216303Documentos Relacionados
- Transformation of Saccharomyces cerevisiae with linear DNA killer plasmids from Kluyveromyces lactis.
- Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character.
- Genome organization of the killer plasmid pGK12 from Kluyveromyces lactis.
- Kluyveromyces lactis killer system: analysis of cytoplasmic promoters of the linear plasmids.
- Transfer of DNA killer plasmids from Kluyveromyces lactis to Kluyveromyces fragilis and Candida pseudotropicalis.
