Cytidine 5'-diphosphate reductase activity in phytohemagglutinin stimulated human lymphocytes.
AUTOR(ES)
Tyrsted, G
RESUMO
The optimal conditions and the effect of deoxyribonucleoside triphosphates were determined for CDP reductase activity in PHA-stimulated lymphocytes. The enzymatic reaction showed an absolute requirement for ATP. In the absence of ATP, only dATP showed a minor stimulation of the reduction of CDP to dCDP. During transformation the CDP reductase activity reached a maximum at the same time as the four deoxyribonucleoside triphosphate pools, corresponding to mid S-phase at about 50 h after PHA addition. The DNA polymerase activity reached a maximum at 57 h.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327690Documentos Relacionados
- Adenosine metabolism in phytohemagglutinin-stimulated human lymphocytes.
- Expression of cell-cycle-dependent genes in phytohemagglutinin-stimulated human lymphocytes.
- Cyclic adenosine 3′,5′-monophosphate in human lymphocytes. Alterations after phytohemagglutinin stimulation
- Relationship between sterol synthesis and DNA synthesis in phytohemagglutinin-stimulated mouse lymphocytes.
- Replication of Poliovirus in Phytohemagglutinin-stimulated Human Lymphocytes
