Cytochemical Localization of K+-stimulated Adenosine Triphosphatase Activity in Xylem Parenchyma Cells of Barley Roots 1
AUTOR(ES)
Winter-Sluiter, Erika
RESUMO
ATPase activity in xylem parenchyma cells of barley (Hordeum vulgare L.) roots was demonstrated cytochemically with a lead precipitation reaction. The methodical parameters of this cytochemical test were optimized for distinction between ATPase-specific and nonspecific precipitates. Optimum conditions were prefixation in 1% glutaraldehyde for 1 hour and incubation for 2 hours in a medium containing 2 mm each of ATP, Ca2+, and Pb2+ at pH 7 and 25 C. Problems of cytochemical localizations are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=542746Documentos Relacionados
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