Cytochemical localization of lipopolysaccharides during peptidoglycan degradation of Escherichia coli cells.
AUTOR(ES)
Frehel, C
RESUMO
The cytochemical reaction of Thiery [J. P. Thiery, J. Microsc. (Paris) 6:987-1018, 1976] was applied to several Escherichia coli strains having different lipopolysaccharide molecular structures. The granular deposit obtained strongly suggested that part of the R core exposed on the outer membrane was responsible for the staining. As this procedure specifically stains the outer membrane, it was possible to demonstrate that, in E. coli K-12, changes in lipopolysaccharide distribution occurred during autolysis and lysozyme treatment.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=211755Documentos Relacionados
- Correlation between degradation and ultrastructure of peptidoglycan during autolysis of Escherichia coli.
- Cytochemical Localization of Certain Phosphatases in Escherichia coli
- Role of ribosome degradation in the death of starved Escherichia coli cells.
- Herpes simplex virus and human cytomegalovirus replication in WI-38 cells. III. Cytochemical localization of lysosomal enzymes in infected cells.
- Immunocytochemical localization of enterobacterial common antigen in Escherichia coli and Yersinia enterocolitica cells.
