Cytochrome c folding pathway: Kinetic native-state hydrogen exchange
AUTOR(ES)
Hoang, Linh
FONTE
National Academy of Sciences
RESUMO
Native-state hydrogen exchange experiments under EX1 conditions can distinguish partially unfolded intermediates by their formation rates and identify the amide hydrogens exposed and protected in each. Results obtained define a cytochrome c intermediate seen only poorly before and place it early on the major unfolding pathway. Four distinct unfolding steps are found to be kinetically ordered in the same pathway sequence inferred before.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=129417Documentos Relacionados
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