Cytoplasmic, Nuclear, and Platelet Autoantibodies in Human Granulocytic Ehrlichiosis Patients
AUTOR(ES)
Wong, Susan J.
FONTE
American Society for Microbiology
RESUMO
Serum samples from patients with confirmed human granulocytic ehrlichiosis (HGE) were tested for cytoplasmic, nuclear, and platelet autoantibodies and rheumatoid factor. The indirect fluorescence antinuclear antibody test on Hep-2 cells demonstrated antinuclear titers of ≥40 and ≥160 in 44 and 10%, respectively, of serum samples from HGE patients. Two patients (4%) had anticytoplasmic (mitochondrial and spindle apparatus) antibodies with a titer of 80 and two patients (4%) had anticytoplasmic (mitochondrial) antibodies with a titer of 160 or greater. Flow cytometry was used to demonstrate antiplatelet antibodies in 80% of first serum samples from HGE patients. Rheumatoid factor was not detected. Nuclear and cytoplasmic autoantibodies are a major cause of interference when the indirect fluorescence antibody test is used to detect fluorescence of morulae in Ehrlichia-infected equine neutrophils or HL-60 promyelocytes. Antiplatelet antibodies may contribute to the profound thrombocytopenia which is a characteristic laboratory feature during the acute phase of HGE infection. Whether autoantibodies precede infection or are caused by immune activation of HGE deserves further study.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=104960Documentos Relacionados
- Human cytomegalovirus UL36-38 and US3 immediate-early genes: temporally regulated expression of nuclear, cytoplasmic, and polysome-associated transcripts during infection.
- Human Granulocytic Ehrlichiosis Agent and Ehrlichia chaffeensis Reside in Different Cytoplasmic Compartments in HL-60 Cells
- Clinical and Serological Follow-Up of Patients with Human Granulocytic Ehrlichiosis in Slovenia
- Rheumatic fever: autoantibodies against a variety of cardiac, nuclear, and streptococcal antigens.
- Serologic and Molecular Detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (Human Granulocytic Ehrlichiosis Agent) in Korean Patients