Cytoplasmic viral DNA synthesis in exogenous infection of murine leukemia virus: effect of interferon and cycloheximide.
AUTOR(ES)
Aboud, M
RESUMO
Cytoplasmic viral DNA synthesis can be followed efficiently by [3H]thymidine labeling of cells exogenously infected with Moloney murine leukemia virus. Both the negative and the positive strands of viral DNA reached their maximal level in the cytoplasm at 3.5 h postinfection. Interferon treatment before infection markedly reduced the amount of viral DNA formed during the first 3.5 h, but led to a second major wave of viral DNA synthesis, peaking at 7.5 h postinfection. No such late cytoplasmic DNA synthesis occurred in the untreated control. Inhibition of protein synthesis by cycloheximide, on the other hand, stimulated cytoplasmic viral DNA synthesis during the first 3.5 h.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=171073Documentos Relacionados
- DNA synthesis in polyoma virus infection. III. Mechanism of inhibition of viral DNA replication by cycloheximide.
- Intracellular production of virus particles and viral components in NIH/3T3 cells chronically infected with Moloney murine leukemia virus: effect of interferon.
- Effect of Fv-1 gene product on synthesis of linear and supercoiled viral DNA in cells infected with murine leukemia virus.
- Moloney Murine Leukemia Virus Integrase Protein Augments Viral DNA Synthesis in Infected Cells
- Low-multiplicity infection of Moloney murine leukemia virus in mouse cells: effect on number of viral DNA copies and virus production in producer cells.
