Dark Recovery Processes in Escherichia coli Irradiated with Ultraviolet Light I. Effect of rec− Mutations on Liquid Holding Recovery1
AUTOR(ES)
Ganesan, Ann K.
RESUMO
We have examined various derivatives of Escherichia coli K-12 for liquid holding recovery, a type of recovery originally observed in E. coli B irradiated with ultraviolet light. Although most of the K-12 derivatives tested showed relatively little or no recovery under our conditions, four of the six independent rec− mutants examined, those carrying recA1, rec-12, recA13, and rec-56, respectively, displayed marked recovery. These mutants are distinguished from rec+ strains by their increased sensitivity to ultraviolet radiation and decreased ability to undergo genetic recombination. Two of them have also been reported to release large amounts of their deoxyribonucleic acid as acid-soluble material, especially after irradiation. None of the three uvr− mutants examined, containing uvrA6, uvrB5, or uvrC34, showed comparable liquid holding recovery. The one rec− uvr− derivative tested, carrying recA13 and uvrA6, did not appear to undergo liquid holding recovery, although recA13 uvr+ strains did. Genetic analysis of one strain, a recA13 mutant, indicated that all the rec+ derivatives obtained from it by conjugation, transduction and reversion, had lost the property of showing liquid holding recovery. From these results, we conclude that in E. coli K-12 the expression of liquid holding recovery depends upon certain rec− mutations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=252307Documentos Relacionados
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