De novo synthesis of the early transcription factor 70-kilodalton subunit is required for morphogenesis of vaccinia virions.

AUTOR(ES)

Hu, X

RESUMO

Vaccinia virus early transcription factor (VETF) is a heterodimeric protein that is packaged in virus particles for expression of early genes during the next round of infection. To investigate additional roles of VETF, we constructed a conditionally lethal recombinant vaccinia virus in which the D6R gene, encoding the 70-kDa subunit of VETF, is under stringent Escherichia coli lac operator control. When cells were infected with the recombinant virus in the absence of an inducer, synthesis of the 70-kDa protein was undetectable and the yield of infectious virus was severely reduced. Under these nonpermissive conditions, DNA replication and synthesis of viral proteins other than the one encoded by D6R occurred, suggesting that de novo synthesis of VETF is not required for expression of early or late genes during the virus growth cycle. Electron microscopy, however, revealed that immature virus particles and masses of electron-dense material accumulated in the absence of an inducer. We concluded that VETF has a direct role in virion morphogenesis or is required for expression of a novel subset of genes that have such a role.

Documentos Relacionados

• Repression of the A8L Gene, Encoding the Early Transcription Factor 82-Kilodalton Subunit, Inhibits Morphogenesis of Vaccinia Virions
• Early promoter-binding factor from vaccinia virions.
• Vaccinia Virus Morphogenesis: A13 Phosphoprotein Is Required for Assembly of Mature Virions
• Transcription of vaccinia virus early genes by a template-dependent soluble extract of purified virions.
• Vaccinia Virus WR Gene A5L Is Required for Morphogenesis of Mature Virions