Decreasing the number of 68-base-pair tandem repeats in the BK virus transcriptional control region reduces plaque size and enhances transforming capacity.

AUTOR(ES)

Watanabe, S

RESUMO

Wild-type BK virus, which has three 68-base-pair (bp) elements tandemly repeated in its transcriptional control region, forms clear, large plaques in human embryonic kidney cell cultures but rarely transforms hamster or rat cells. From this BK virus DNA, deletion mutants with fewer than three 68-bp elements were constructed and characterized. The mutant DNA with two 68-bp elements formed small, turbid plaques in human cell cultures and transformed rat 3Y1 cells inefficiently. The mutant DNA with one 68-bp element formed minute, turbid plaques in human cell cultures and transformed rat cells efficiently.

Documentos Relacionados

• Enhancement of the transforming capacity of BK virus by partial deletion of the 68-base-pair tandem repeats.
• The 5'-limit of transposition and upstream barren region of a trypanosome VSG gene: tandem 76 base-pair repeats flanking (TAA)90.
• Evolutionary changes of transcriptional control region in a minute-plaque viable deletion mutant of BK virus.
• A 36-base-pair core sequence of locus control region enhances retrovirally transferred human beta-globin gene expression.
• Human T-cell lymphotropic virus type I (HTLV-I) transcriptional activator, Tax, enhances CREB binding to HTLV-I 21-base-pair repeats by protein-protein interaction.