Defective Bacteriophage PBSH in Bacillus subtilis: III. Properties of Adenine-16 Marker in Purified Bacteriophage Deoxyribonucleic Acid
AUTOR(ES)
Haas, Martin
RESUMO
The adenine-16 (ade-16) marker (the marker nearest the chromosomal origin of Bacillus subtilis) in purified PBSH deoxyribonucleic acid (DNA) renatured more rapidly and to a greater extent than any other marker in the phage DNA, and more rapidly and to a greater extent than all markers, including ade-16, in bacterial DNA. The renaturation of the phage DNA ade-16 marker followed a first-order reaction, whereas renaturation of bacterial markers was initially a second-order reaction. No cross-linkages were detected in DNA molecules containing the ade-16 marker. Buoyant density measurements and inactivation by heat and micrococcal deoxyribonuclease of the ade-16 marker did not reveal large segments of clusters of the individual bases in these molecules. Alternative mechanisms for the unique renaturation behavior of the ade-16 marker are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=375947Documentos Relacionados
- Defective Bacteriophage PBSH in Bacillus subtilis: I. Induction, Purification, and Physical Properties of the Bacteriophage and Its Deoxyribonucleic Acid
- Defective Bacteriophage PBSH in Bacillus subtilis: II. Intracellular Development of the Induced Prophage
- Genetic recombination during transformation in Bacillus subtilis: appearance of a deoxyribonucleic acid methylase.
- Thymineless Death in Bacillus subtilis: Correlation Between Cell Lysis and Deoxyribonucleic Acid Breakdown
- Thymineless Death in Bacillus subtilis: Correlation Between Cell Lysis and Deoxyribonucleic Acid Breakdown
