Dehalogenation of Dichloromethane by Dichloromethane Dehalogenase/Glutathione S-Transferase Leads to Formation of DNA Adducts
AUTOR(ES)
Kayser, Martin F.
FONTE
American Society for Microbiology
RESUMO
Formation of DNA adducts following conversion of dichloromethane by bacterial dichloromethane dehalogenase/glutathione S-transferase was demonstrated. Adducts included dichloromethane carbon and glutathione sulfur atoms. A reaction with DNA occurred preferentially at guanine bases. Increased DNA degradation in a polA mutant of Methylobacterium dichloromethanicum DM4 grown with dichloromethane confirmed the genotoxicity associated with dichloromethane degradation, suggesting an important role of DNA repair in the metabolism of halogenated, DNA-alkylating compounds by bacteria.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=95400Documentos Relacionados
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