Deletion and Amber Mutants of fla Loci in ESCHERICHIA COLI K-12

AUTOR(ES)

Kondoh, Hisato

RESUMO

A rapid screening method for amber fla mutants of E. coli was devised and many mutants were obtained. In addition, strains with deletions of the fla genes in the his-uvrC region were isolated from high-temperature survivors of a λcI857 lysogen in which the prophage is located between his and fla. Utilizing these mutants, eleven fla genes (I—XI) and one hag gene were identified in the his-uvrc region, in the following order: his-supD-I-II-(III, IV)-V-(VI, VII)-VIII-IX-hag-(X, XI)-uvrC. The fla genes X and XI and hag are located at about 42.5 min and the other fla genes at about 43.0 min on the E. coli genetic map (Bachmann, Low and Taylor 1976). Mutants of fla gene X showed a slight sensitivity to chi phage, although they lack the flagellar filament.

Documentos Relacionados

• Potassium Transport Loci in Escherichia coli K-12
• Flavodoxin Mutants of Escherichia coli K-12
• Phosphoglucomutase Mutants of Escherichia coli K-12
• Malate Dehydrogenase Mutants in Escherichia coli K-12
• Bacteriophage chi sensitivity and motility of Escherichia coli K-12 and Salmonella typhimurium Fla- mutants possessing the hook structure.