Deletions fusing the hisG and hisD genes in Salmonella typhimurium.
AUTOR(ES)
Ino, I
RESUMO
Frameshift mutation hisD497 occurs in the operator-proximal portion of the Salmonella typhimurium gene coding for the dimeric protein, L-histidinol dehydrogenase (HDH). Rare revertants of hisD497 are deletions fusing the hisD gene to the adjacent preceding structural gene, hisG (adenosine 5'-triphosphate-PR transferase). HDH purified from one revertant, hisGD4908, contains subunits of approximately normal molecular weight but with no clearly demonstrable unique amino-terminal sequence. We propose that a combined inactive G-D polypeptide is synthesized and then cleaved at a number of closely juxtaposed sites by endoproteolytic activity. At least some of the resulting fragments then participate in formation of active HDH dimers.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=235849Documentos Relacionados
- Features of the rho-dependent transcription termination polar element within the hisG cistron of Salmonella typhimurium.
- A Refined Map of the hisG Gene of SALMONELLA TYPHIMURIUM
- Nature of the hisD3018 Frameshift Mutation in Salmonella typhimurium
- Reversion of the Frameshift hisD3018 of Salmonella typhimurium
- Histidinol Dehydrogenase (hisD) Mutants of Salmonella typhimurium1