Demonstration of an iron-siderophore-binding protein in the outer membrane of Pseudomonas aeruginosa.
AUTOR(ES)
Sokol, P A
RESUMO
Outer membranes prepared from Pseudomonas aeruginosa grown in low-iron medium bind three times as much [59Fe]pyochelin as do membranes from cells grown in high-iron medium. The deletion of pyochelin reduced 59Fe binding to background levels. Autoradiographic analysis of sodium dodecyl sulfate polyacrylamide gel electrophoretograms of outer membrane preparations previously incubated with [59Fe]pyochelin revealed that the iron-siderophore complex bound to a low-molecular-weight protein of approximately 14,000.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=264906Documentos Relacionados
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