Demonstration of high molecular weight poly (adenosine diphosphate ribose).

AUTOR(ES)

Tanaka, M

RESUMO

An electrophoretic system was established that resolves poly(adenosine diphosphate ribose), enzymatically synthesized polymer from NAD+, by size difference of one residue on polyacrylamide gel. The existence of a polymer of at least 65 residues was demonstrated by band counting in this system. The polymer showed a heterogeneous size distribution on the electrophoregram, and the molecular weight of the largest polymer was deduced to be more than 4.5 X 10(5) daltons. The discrepancy between the size, estimated by electrophoresis, and the chain length, determined by the ratio of total radioactivity to that derived from the terminus, suggests that the polymer has a branched structure.

Documentos Relacionados

• Natural occurence of a biopolymer, poly (adenosine diphosphate ribose).
• Studies on autoantibodies to poly (adenosine diphosphate-ribose) in SLE and other autoimmune diseases.
• Formation and characterization of antibody against 2'-(5"-phosphoribosyl)-5' AMP, the monomer form of poly(adenosine diphosphate ribose).
• Human autoantibodies to poly(adenosine diphosphate-ribose) polymerase.
• Antibody to poly(adenosine diphosphate-ribose) polymerase and its use in chromatin analysis.