Demonstration of subpopulations of Dane particles.
AUTOR(ES)
Kaplan, P M
RESUMO
Two populations of Dane particles were isolated from the plasma of individuals carrying hepatitis B surface antigen. These populations had densities in CsCl of 1.22 and 1.20 g/ml. Endogenous DNA polymerase activity was found to be associated only with the heavier of these two populations. Using a positive stain, electron microscopic examination of these particles suggested that the heavier the particle contained nucleic acid in its core whereas the lighter particle appeared empty. Cores isolated from Dane particles with densities of 1.22 and 1.20 g/ml banded in CsCl at densities of 1.36 and 1.30 g/ml, respectively. Endogenous DNA polymerase activity was associated only with the higher density core particles.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=515489Documentos Relacionados
- Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles.
- Three envelope proteins of hepatitis B virus: large S, middle S, and major S proteins needed for the formation of Dane particles.
- Distinct deoxyribonucleic acid polymerase activities associated with Dane particles and naked Dane cores.
- Chemically synthesized peptides predicted from the nucleotide sequence of the hepatitis B virus genome elicit antibodies reactive with the native envelope protein of Dane particles.
- Inhaled Particles. III