DeoR repression at-a-distance only weakly responds to changes in interoperator separation and DNA topology.
AUTOR(ES)
Dandanell, G
RESUMO
The interoperator distance between a synthetic operator Os and the deoP2O2-galK fusion was varied between 46 and 176 bp. The repression of the deoP2 directed galK expression as a function of the interoperator distance (center-to-center) was measured in vivo in a single-copy system. The results show that the DeoR repressor efficiently can repress transcription at all the interoperator distances tested. The degree of repression depends very little on the spacing between the operators, however, a weak periodic dependency of 8-11 bp may exist.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=334349Documentos Relacionados
- Two operator sites separated by 599 base pairs are required for deoR repression of the deo operon of Escherichia coli.
- Identification of the Escherichia coli deoR and cytR gene products.
- Purification and Characterization of the DeoR Repressor of Bacillus subtilis
- Purification and characterization of the deoR repressor of Escherichia coli.
- The primary structure of the DeoR repressor from Escherichia coli K-12.