Deoxyribonucleic acid sequence of araBAD promoter mutants of Escherichia coli.
AUTOR(ES)
Horwitz, A H
RESUMO
The controlling site region for the araBAD operon is defined, in part, by two classes of cis-acting constitutive mutations. The aralc mutations allow low-level constitutive expression of ara-BAD in the absence of the positive regulatory protein coded for by the araC gene, whereas the araXc mutations allow expression of araBAD in the absence of the cyclic adenosine monophosphate receptor protein. Six independently isolated aralc mutations and three independently isolated araXc mutations were cloned onto the plasmid pBR322 using in vitro recombinant deoxyribonucleic acid techniques and in vivo recombination between plasmid and chromosomal deoxyribonucleic acid. The location of these mutations was determined by deoxyribonucleic acid sequence analysis. All of the aralc mutations occurred at position -35 within the araBAD promoter (+1 = messenger ribonucleic acid start for araBAD) and resulted from an AT leads to GC transition. All of the araXc mutations occurred at position -10 within the araBAD promoter and resulted from a GC leads to AT transition. Models are presented to explain the mode of action of the aralc and araXc mutations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=294045Documentos Relacionados
- DNA sequence of the araBAD promoter in Escherichia coli B/r.
- Five mutations in the promoter region of the araBAD operon of Escherichia coli B/r.
- Nucleotide sequence of the 5' end of araBAD operon messenger RNA in Escherichia coli B/r.
- Regulation of the araC gene of Escherichia coli: catabolite repression, autoregulation, and effect on araBAD expression.
- Arabinose-induced binding of AraC protein to araI2 activates the araBAD operon promoter.
