Detection and characterization of epithelial cells in the peripheral blood of patients with breast cancer / Detecção e caracterização de células epiteliais no sangue periférico de pacientes com carcinoma localmente avançado de mama

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

The aim of this study was to detect and characterize carcinoma cells of patients with breast cancer with clinical stage III analyzing the presence or absence before chemotherapy by enrichment of peripheral blood with super paramagnetic microbeads, using direct Immunomagnetic labeling of intracellular 7/8 cytokeratin. For enrichment, magnetically labeled tumor cells are passed over a high-gradient magnetic positive selection column. Cytospin preparations are made after immunomagnetic enrichment and are examined immunocytochemically (ICC) using an anti-cytokeratin and quantified by flow cytometry (CMF). Results: No cytokeratin positive cells were detected in the 32 peripheral blood of patients of breast carcinoma by ICC and cytokeratin positive cells were detected in the 22 of 23 patients by CMF. Conclusion: These methods of detection and characterization breast carcinoma cells will become useful in the diagnosis and prognosis but is necessary optimization of existing methods.

ASSUNTO(S)

keratins imunocitoquímica separação imunomagnética breast neoplasms immunohistochemistry flow cytometry neoplasia mamária citometria de fluxo immunomagnetic separation queratina

ACESSO AO ARTIGO

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