Detection and ultrastructural localization of human smooth muscle myosin-like molecules in human non-muscle cells by specific antibodies.
AUTOR(ES)
Painter, R G
RESUMO
Spectrin, a protein complex which is peripherally attached to the cytoplasmic surface of the human erythrocyte membrane, cannot be detected (by complement fixation with anti-spectrin antibodies) in homogenates of several different human non-muscle cells studied. On the other hand, a protein antigenically identical or similar to human smooth muscle myosin was detected (by complement fixation with antibodies to uterine smooth muscle myosin) in these cells. In the case of human fibroblast line WI38, this smooth muscle myosin like component was shown (by ferritin-antibody experiments in electron microscopy) to be at least partly associated with cytoplasmic surface of the plasma membrane of the cell. It is proposed that the spectrin complex of the erythrocyte membrane and the smooth muscle myosin-like component of the fibroblast membrane play similar roles in regulating the translational mobilities of integral proteins in their respective membranes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=432533Documentos Relacionados
- The heavy chain of Acanthamoeba myosin IB is a fusion of myosin-like and non-myosin-like sequences.
- Filamin, a new high-molecular-weight protein found in smooth muscle and non-muscle cells.
- Actin Antibody: The Specific Visualization of Actin Filaments in Non-Muscle Cells
- Characterization of antibodies to smooth muscle myosin kinase and their use in localizing myosin kinase in nonmuscle cells.
- Alpha-actinin synthesis can be modulated by antisense probes and is autoregulated in non-muscle cells.