Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe.
AUTOR(ES)
Subramanian, K
RESUMO
A nucleic acid hybridization assay was developed to rapidly detect small quantities of aquareovirus RNAs in infected cells and organs. Cloned cDNA copies were synthesized from the genomic RNA of the SBR strain of aquareovirus. By using cloned cDNA probes, aquareovirus RNAs were detected in spleen and kidney tissues of experimentally infected fish.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=265588Documentos Relacionados
- Detection of group B rotavirus in fecal specimens by dot hybridization with a cloned cDNA probe.
- Detection of a unique human V kappa IV germline gene by a cloned cDNA probe.
- Detection of proopiomelanocortin mRNA by in situ hybridization with an oligonucleotide probe.
- Detection of DNA "fingerprints" of cultivated rice by hybridization with a human minisatellite DNA probe.
- cDNA cloning of a novel heterogeneous nuclear ribonucleoprotein gene homologue in Caenorhabditis elegans using hamster prion protein cDNA as a hybridization probe.