Detection of Aspergillus fumigatus and a Mutation That Confers Reduced Susceptibility to Itraconazole and Posaconazole by Real-Time PCR and Pyrosequencing
AUTOR(ES)
Trama, Jason P.
FONTE
American Society for Microbiology
RESUMO
A real-time PCR and pyrosequencing method was developed to detect Aspergillus fumigatus in whole blood by amplifying the cyp51A gene and sequencing the codon for glycine 54. Mutations in this codon can result in amino acid substitutions that confer reduced susceptibility to itraconazole and posaconazole.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=548089Documentos Relacionados
- Rapid, High-Throughput, Multiplex, Real-Time PCR for Identification of Mutations in the cyp51A Gene of Aspergillus fumigatus That Confer Resistance to Itraconazole
- Semiquantitative Detection by Real-Time PCR of Aspergillus fumigatus in Bronchoalveolar Lavage Fluids and Tissue Biopsy Specimens from Patients with Invasive Aspergillosis
- Quantitative Detection of Borrelia burgdorferi by Real-Time PCR
- Detection and Differentiation of Cryptosporidium Parasites That Are Pathogenic for Humans by Real-Time PCR
- Detection of Yersinia pestis in Sputum by Real-Time PCR