Detection of bacteria in the presence of antibiotics by using specific monoclonal antibodies to neutralize the antibiotics.

AUTOR(ES)

Sierra-Madero, J G

RESUMO

Inactivation of penicillin and gentamicin in cultures was achieved by using monoclonal antibodies against these antibiotics. A viridans group streptococcus (penicillin MIC, less than or equal to 0.06 micrograms/ml) and Escherichia coli ATCC 35218 (gentamicin MIC, less than or equal to 1 microgram/ml) were able to grow in broth containing 0.25 micrograms of penicillin per ml and 4 micrograms of gentamicin per ml, respectively, when the specific antibodies were added. This procedure may be useful to increase the yield of bacteria from body fluid specimens that contain antibiotics.

Documentos Relacionados

• Inhibition of adherence of mucoid Pseudomonas aeruginosa by alginase, specific monoclonal antibodies, and antibiotics.
• Polycations sensitize enteric bacteria to antibiotics.
• Detection of specific immunoglobulin M antibodies to cytomegalovirus by using monoclonal antibody to immunoglobulin M in an indirect immunofluorescence assay.
• Detection of enzyme polymorphism by using monoclonal antibodies.
• Susceptibilities of anaerobic bacteria to cefoperazone and other antibiotics.